Info_Biomedicina_Genomica_secuenciacion_servicios_secuenciacion_sanger

SEQUENCING SANGER and NGS

  • SANGER:
    • Standard amplification reactions (PCR).
    • Purification and quantification of PCR products.
    • Sanger sequencing.
    • Capillary electrophoresis (SeqStudio from ThermoFisher).
  • NGS:
    • Generation of NGS libraries.
    • Sequencing on MiSeq (Illumina).
    • Sequencing on NextSeq550 (Illumina)
    • Sequencing on NovaSeq6000 (Illumina)