SEQUENCING SANGER and NGS
- SANGER:
- Standard amplification reactions (PCR).
- Purification and quantification of PCR products.
- Sanger sequencing.
- Capillary electrophoresis (SeqStudio from ThermoFisher).
- NGS:
- Generation of NGS libraries.
- Sequencing on MiSeq (Illumina).
- Sequencing on NextSeq550 (Illumina)
- Sequencing on NovaSeq6000 (Illumina)