GENOTYPE of genomic DNA fragments: STRs, SNPs, indels, ...
- STRs, indels and other types of genomic DNA fragments (STR Code):
- Standard amplification reactions (PCR, multiplex PCR) and Whole Genome Amplification (WGA). (PCR code).
- Capillary electrophoresis -> Fragment genotyping, STRs and indels (see document).
- Determination of size of fluorescently labeled DNA amplified (fragments of genomic DNA).
- Microsatellite multiplex genotyping (STRs): simultaneous detection of up to 5 colors.
- SNPs and Indels (SNP Code):
- BioMark HD System - Fluidigm (multiplex of 12-48-96 SNPs vs samples) (see document).
- TaqMan - single assays (1 SNP per reaction, RT-PCR analysis in equipment CFX96) (see document).
- HRMA (High Resolution Melt Analysis) in equipment CFX96
- SNaPshot (1-20 SNPs by multiple PCR and capillary migration detection) (see document).