Alicia G. Gubieda, Lucía Gandarias, Mihály Pósfai, Ajith Pattammattel, M. Luisa Fdez-Gubieda, Ana Abad-Díaz-de-Cerio and Ana García-Prieto
Journal of Nanobiotechnology, Volume 22, article number 529, (2024)
Magnetic nanoparticles offer many exciting possibilities in biomedicine, from cell imaging to cancer treatment. One of the currently researched nanoparticles are magnetosomes, magnetite nanoparticles of high chemical purity synthesized by magnetotactic bacteria. Despite their therapeutic potential, very little is known about their degradation in human cells, and even less so of their degradation within tumours. In an effort to explore the potential of magnetosomes for cancer treatment, we have explored their degradation process in a 3D human lung carcinoma model at the subcellular level and with nanometre scale resolution. We have used state of the art hard X-ray probes (nano-XANES and nano-XRF), which allow for identification of distinct iron phases in each region of the cell. Our results reveal the progression of magnetite oxidation to maghemite within magnetosomes, and the biosynthesis of magnetite and ferrihydrite by ferritin.
Danny Villanueva, Alicia G. Gubieda, Lucía Gandarias, Ana Abad Díaz de Cerio, Iñaki Orue, José Ángel García, David de Cos, Javier Alonso, M. Luisa Fdez-Gubieda
ACS Applied Materials & Interfaces, Vol 16/Issue 49, 2024
Magnetotactic bacteria have been proposed as ideal biological nanorobots due to the presence of an intracellular chain of magnetic nanoparticles (MNPs), which allows them to be guided and controlled by external magnetic fields and provides them with theragnostic capabilities intrinsic to magnetic nanoparticles, such as magnetic hyperthermia for cancer treatment. Here, we study three different bacterial species, Magnetospirillum gryphiswaldense (MSR-1), Magnetospirillum magneticum (AMB-1), and Magnetovibrio blakemorei(MV-1), which synthesize magnetite nanoparticles with different morphologies and chain arrangements. We analyzed the impact of these parameters on the effective magnetic anisotropy, Keff, and the heating capacity or Specific Absorption Rate, SAR, under alternating magnetic fields. SAR values have been obtained from the area of experimental AC hysteresis loops, while Keff has been determined from simulations of AC hysteresis loops using a dynamic Stoner–Wohlfarth model. The results demonstrate a clear relationship between the effective magnetic anisotropy and the heating efficiency of bacteria. As the Keff value increases, the saturated SAR values are higher; however, the threshold magnetic field required to observe a SAR response simultaneously increases. This factor is crucial to choose a bacterial species as the optimal hyperthermia agent.
Antibacterial performance of Co–Zn ferrite nanoparticles under visible light irradiation
Alicia G. Gubieda, Ana Abad-Díaz-de-Cerio, Ana García-Prieto, M. Luisa Fdez-Gubieda, Laura Cervera-Gabalda, Eduardo Ordoqui-Huesa, Alfonso Cornejo, Cristina Gómez-Polo
Journal of Chemical Technology and Biotechnology, (2024)
To address water scarcity and promote sustainable resource management, more efficient and cost-effective water treatment solutions are necessary. Particularly, pathogens in drinking water are a topic of growing concern. One promising technology is the use of photocatalytic nanoparticles activated by visible light as antibacterial agents. This study focuses on the characterization and antibacterial properties of Co–Zn ferrite nanocatalysts, tested against Escherichia coli.
Publications 2013-2023
Intracellular biotransformation and disposal mechanisms of magnetosomes in macrophages and cancer cells, Lucia Gandarias,Alicia G. Gubieda, Giulio Gorni, Olivier Mathon, Luca Olivi, Ana Abad-Díaz-de-Cerio, MaLuisa Fdez-Gubieda, Alicia Muela, and Ana Garcia-Prieto, Biotechnology Journal (2023)
Incorporation of Tb and Gd improves the diagnostic functionality of magnetotactic bacteria, L. Gandarias, E.M. Jefremovas, D. Gandia, L. Marcano, V. Martínez-Martínez, P. Ramos-Cabrer, Daniel M. Chevrier, S. Valencia, L. Fernández Barquín, M.L. Fdez-Gubieda, J. Alonso, A. García-Prieto, A. Muela; Materials Today Bio, 20, 2023, 100680
Tuning the Magnetic Response of Magnetospirillum magneticum by Changing the Culture Medium: A Straightforward Approach to Improve Their Hyperthermia Efficiency; D. Gandia, L. Marcano, L. Gandarias, D. Villanueva, I. Orue, R. Abrudan, S. Valencia, I. Rodrigo, J.A. García, A. MIuela, M.L. Fdez-Gubieda, J. Alonso, ACS Appl. Mater. Interfaces 2023, 15, 1, 566–577
Magnetic Anisotropy of Individual Nanomagnets Embedded in Biological Systems Determined by Axi-asymmetric X‑ray Transmission Microscopy; Lourdes Marcano, Iñaki Orue, David Gandia, Lucía Gandarias, Markus Weigand, Radu Marius Abrudan, Ana García-Prieto, Alfredo García-Arribas, Alicia Muela, M. Luisa Fdez-Gubieda, and Sergio Valencia, ACS Nano 2022, 16, 7398−7408
Towards the design of contrast-enhanced agents: systematic Ga3+ doping on magnetite nanoparticles, ltziar Galarreta-Rodriguez, Lourdes Marcano, ldoia Castellanos-Rubio, lzaskun Gil de Muro, Isabel García, Luca Olivi, M. L. Fernández-Gubieda, Ainara Castellanos-Rubio, Luis Lezama, ldoia Ruiz de Larramendi and Maite lnsausti. Dalton Transactions (2022) 51, 2517-2530
Modifying the magnetic response of magnetotactic bacteria: incorporation of Gd and Tb ions into the magnetosome structure; E.M. Jefremovas, L. Gandarias, L. Marcano, A. García-Prieto, I. Orue, A. Muela, M. L. Fdez-Gubieda, L. Fernández Barquín, and J. Alonso*. Nanoscale Adv.,4, 2649-2659 (2022)
Shaping Up Zn-Doped Magnetite Nanoparticles from Mono- and Bimetallic Oleates: The Impact of Zn Content, Fe Vacancies, and Morphology on Magnetic Hyperthermia Performance, Idoia Castellanos-Rubio, Oihane Arriortua, Lourdes Marcano, Irati Rodrigo, Daniela Iglesias, Ander Barón, Ane Olazagoitia-Garmendia, Luca Olivi, Fernando Plazaola, M. luisa Fdez-Gubieda, Ainara Castellanos-Rubio, José S. Garitaonandia, Iñaki Orue, and Maite Insausti, Chemistry of Materials, (2021), 33, 9, 3139–3154
Nanoflowers vs. Magnetosomes: Comparison between two promising candidates for Magnetic Hyperthermia Therapy, E. M. jefremovas, L. Gandarias, I. Rodrigo, l. Marcano, C. Grüttner, J. A. García, E. Garaio, I. Orue, A. García-Prieto, A. Muela, M. L. Fdez-Gubieda, J. Alonso and L. Fernández Barquín, IEEE Access Journal, (2021)
A Milestone in the Chemical Synthesis of Fe3O4 Nanoparticles: Unreported Bulk-like Properties lead to a Remarkable Magnetic Hyperthermia Castellanos-Rubio, I; Arriortua, O; Barón, A; Iglesias, D; Rodrigo, I; Marcano, L.; José S. Garitaonandia, Iñaki Orue, M. Luisa Fdez-Gubieda, and Maite Insausti; Chemistry of Materials, 2021, 33 (22) 8693-8704.
Improved photocatalytic and antibacterial performance of Cr doped TiO2 nanoparticles, C. Gomez-Polo, S. Larumbe, A. Gil, D. Muñoz, L. Rodríguez Fernández, L. Fernández Barquín, A. García-Prieto, M.L. Fdez-Gubieda, A. Muela, Surfaces and Interfaces 22 (2021) 100867
Controlled magnetic anisotropy in single domain Mn-doped biosynthesized magnetite nanoparticle, Lourdes Marcano, IñakiOrue, AnaGarcía-Prieto, Radu Abrudan, JavierAlonso, Luis Fernańdez Barquín, Sergio Valencia, Alicia Muela and M. Luisa Fdez-Gubieda, J. Phys. Chem. C 2020, 124, 22827−22838
Investigating the Size and Microstrain Influence in the Magnetic Order/Disorder State of GdCu2 Nanoparticles, EM Jefremovas, J Alonso, M De la Fuente Rodríguez, J Rodríguez Fernández, JI Espeso, DP Rojas, Ana García-Prieto, ML Fernández-Gubieda, L Fernández Barquín, Nanomaterials 10(6) (2020) 1117
Exploring the different degrees of magnetic disorder in TbxR1-xCu2 nanoparticle alloys, Elizabeth M Jefremovas, María de la Fuente Rodríguez, Javier Alonso Masa, Jesús Rodríguez Fernández, Jose Ignacio Espeso Martínez, Inés Puente-Orench, Daniel Rojas Pupo, Ana García Prieto, María Luisa Fernández-Gubieda, Lidia Rodríguez Fernández, Luis Fernández Barquín, Nanomaterials 2020, 10(11), 2148
Isolation of Cancer-Derived Exosomes Using a Variety of Magnetic Nanostructures: From Fe3O4 Nanoparticles to Ni Nanowires, Zohreh Nemati, Mohammad Reza Zamani Kouhpanji, Fang Zhou, Raja Das, Kelly Makielski, Joseph Um, Manh-Huong Phan, Alicia Muela, Mª Luisa Fdez-Gubieda, Rhonda R. Franklin, Bethanie J. H. Stadler, Jaime F. Modiano and Javier Alonso, Nanomaterials 2020, 10, 1662
Perspectives on Magnetotactic bacteria for cancer therapy (invited), Mª Luisa Fernández-Gubieda, J. Alonso, A. García-Prieto, A. García-Arribas, L. Fernandez Barquín, A. Muela, Journal of Applied Physics 128, 070902 (2020)
Magnetosomes could be protective shields against metal stress in magnetotactic bacteria, D. Muñoz, L. Marcano, R. Martín-Rodríguez, L. Simonelli, A. Serrano, A. García-Prieto, M. L. Fdez-Gubieda, A. Muela, Scientific Reports, 10, (2020) 11430
Highly Reproducible Hyperthermia Response in Water, Agar and Cellular Environment by Discretely PEGylated Magnetite Nanoparticles Castellanos-Rubio, Idoia; Rodrigo, Irati; Olazagoitia-Garmendia, Ane; Arriortua, Oihane; Gil de Muro, Izaskun; Garitaonandia, José S.; Bilbao, Jose Ramón; Fdez-Gubieda, Mª Luisa; Plazaola, Fernando; Orue, Iñaki; Castellanos-Rubio, Ainara; Insausti, Maite, ACS Applied Materials & Interfaces 2020, 12, 25, 27917–27929
Elucidating the role of shape anisotropy in faceted magnetic nanoparticles using biogenic magnetosomes as a model, David Gandia; Lucía Gandarias, Lourdes Marcano, Iñaki Orue, David Gil-Cartón, Javier Alonso, Alfredo García-Arribas, Alicia Muela, Mª Luisa Fdez-Gubieda, Nanoscale 2020 12, 16081-16090
Disc-shaped magnetic particles for cancer therapy, M. Goiriena-Goikoetxea, D. Muñoz, I. Orue, M.L. Fernandez-Gubieda, J. Bokor, A. Muela, A. García-Arribas; Applied Physics Review (2020) 7, 011306 (2020)
Study of the Influence of Sensor Permeability in the Detection of a Single Magnetotactic Bacterium, D. De Cos, N. Lete, M.L. Fdez-Gubieda, A. Garcia Arribas, Journal of magnetism and Magnetic Materials, 500 (2020) 166346
Mn-Doping Level Dependence on the Magnetic Response of MnxFe3-xO4 Ferrite Nanoparticles, X. Lasheras, M. Insausti, J. Martínez de la Fuente, I. Gil de Muro, I. Castellanos-Rubio, L. Marcano, M.L. Fdez-Gubieda, A. Serrano, R. Martín-Rodríguez, E. Garaio, J.A. García, L. Lezama, Dalton Transactions 48 (2019) 11480-11491
Unlocking the Potential of Magnetotactic Bacteria as Magnetic Hyperthermia Agents; D. Gandia, L. Gandarias, I. Rodrigo, J. Robles-García, R. Das, E. Garaio, J.Á. García, M.H. Phan, H. Srikanth, I. Orue, J. Alonso, A. Muela, M.L. Fdez-Gubieda, Small 2019, 1902626
Enhanced mass sensitivity in novel magnetoelastic resonators geometries for advanced detection systems, Paula G. Saiza, David Gandia Andoni Lasheras, Ariane Sagasti, Iban Quintana, María Luisa Fdez-Gubieda, Jon Gutiérrez María Isabel Arriortua, Ana Catarina Lopes, Sensors & Actuators: B. Chemical 296 (2019) 126612
Magnetic Study of Co-Doped Magnetosome Chains, Marcano, Lourdes; Muñoz, David; Martín-Rodríguez, Rosa; Orue, Iñaki; Alonso, Javier; García-Prieto, Ana; Serrano, Aida; Valencia, Sergio; Abrudan, Radu; Fernandez Barquin, Luis; García-Arribas, Alfredo; Muela, Alicia; Fdez-Gubieda, Mª Luisa; The Journal of Physical Chemistry, 2018, 122, 7541-7550
Configuration of the magnetosome chain: a natural magnetic nanoarchitecture, I. Orue, L. Marcano, P. Bender, A. García-Prieto, S. Valencia, M.A. Mawass,D. Gil-Cartón,7 D. Alba Venero, D. Honecker, A. García-Arribas, L. Fernández Barquín, A. Muela, M.L. Fdez-Gubieda; Nanoscale, 2018, 10, 7407-7419
Magnetization reversal in circular vortex dots of small radius, Maite Goiriena-Goikoetxea, Konstantin Guslienko, Mikel Rouco, Inaki Orue, Eider Berganza, Miriam Jaafar, Agustina Asenjo, Maria Fdez-Gubieda, Luis Fernandez Barquin and Alfredo García-Arribas, Nanoscale, 2017,9, 11269-11278
Influence of the bacterial growth phase on the magnetic properties of magnetosomes synthesized by Magnetospirillum gryphiswaldense; L. Marcano, A. García-Prieto, D. Muñoz, L. Fernández Barquín, I. Orue, J. Alonso, A. Muela, M.L. Fdez-Gubieda; Biochimica et Biophysica Acta, Volume 1861, Issue 6, June 2017, Pages 1507-1514
Optimal Parameters for Hyperthermia Treatment Using Biomineralized Magnetite Nanoparticles: Theoretical and Experimental Approach; Alicia Muela, David Muñoz, Rosa Martín-Rodríguez, Iñaki Orue, Eneko Garaio, Ana Abad Díaz de Cerio, Javier Alonso, José Ángel García, and M. Luisa Fdez-Gubieda; J. Phys. Chem. C 2016, 120, 24437-24448
Studying nanoparticle’s 3D shape by aspects maps: determination of morphology of bacterial magnetic nanoparticles, D. Peddis, G. Muscas, R, Mathieu, P. Anil Kumar, G. Varvaro, G. Singh, I. Orue, D. Gil-Carton, L. Marcano, A. Muela, M. L. Fdez-Gubieda, Faraday Discussion (2016)
On the mineral core of ferritin-like proteins: structural and magnetic characterization; García-Prieto, Ana; Alonso, J; D. Muñoz; L. Marcano, A. Abad Díaz de Cerio, R. Fernández de Luis, Orue, Inaki; O. Mathon; A. Muela; Fernandez-Gubieda, Maria Luisa, Nanoscale (2016) vol. 8 pp. 1088 (Impact Factor: 7.394)
Assemblies of magnetite nanoparticles extracted from magnetotactic bacteria: a magnetic study; A. M. Huízar-Felix, D. Muñoz, I. Orue, C. Magén, A. Ibarra, J. M. Barandiarán, A. Muela, and M. L. Fdez-Gubieda; APPLIED PHYSICS LETTERS 108, 063109 (2016)
Enhanced magnetic anisotropy and heating efficiency in multi-functional manganese ferrite/graphene oxide nanostructures, Anh-Tuan Le, Chu Duy Giang, Le Thi Tam, Ta Quoc Tuan, Vu Ngoc Phan, Javier Alonso, Jagannath Devkota, Eneko Garaio, José Ángel García, Rosa Martín-Rodríguez, Ma Luisa Fdez-Gubieda, Hariharan Srikanth and Manh-Huong Phan, Nanotechnology 27 (2016) 155707
Magnetic nanoscopic correlations in the crossover between a superspin glass and a superferromagnet, Diego Alba-Venero, Fernandez Barquín Luis, Fdez-Gubieda M.L., Svalov, Alonso Masa Javier, Sean Langridge, Sarah Rogers, J. Applied Physics (2016)
Search for magnetite nanoparticles in the rats'brain, J M Barandiaran, L Martínez-Millán, I Gerrikagoitia, S Orue, I Orue, L Lezama, A. Muela and ML Fernández-Gubieda; IEEE TRANSACTIONS ON MAGNETICS, VOL. 51, NO. 1, JANUARY 2015
High-magnetic field characterization of magnetocaloric effect in FeZrB(Cu) amorphous ribbons, P. Alvarez-Alonso, J. L. Sánchez Llamazares, C. F. Sánchez-Valdés, M.L. Fdez-Gubieda, P. Gorria, and J. A. Blanco; J. Apl. Phys. 117, 17A710 (2015)
Magnetocaloric properties of rapidly solidified Dy3Co alloy ribbons, J.L Sanchez Llamazares, H. Flores-Zúñiga, P. Álvarez-Alonso, C.F. Sánchez-Valdés, G.A. Lara Rodríguez, M.L. Fernández-Gubieda, Journal of Applied Physics 05/2015; 117(17):17A706.
Anisotropy effects in magnetic hyperthermia: A comparison between spherical and cubic exchange-coupled FeO/Fe3O4 nanoparticles H. Khurshid, J. Alonso, Z. Nemati, M. H. Phan, P. Mukherjee, M. L. Fdez-Gubieda, J. M. Barandiarán, and H. Srikanth, Journal of Applied Physics 05/2015; 117, 17A337 (2015).
Breakdown of magnetism in sub-nanometric Ni clusters embedded in Ag; García-Prieto, Ana; Arteche, Alberto; Aguilera-Granja, Faustino; Torres, M; Orue, Inaki; Alonso, J; Fernandez Barquin, Luis; Fernandez-Gubieda, Maria Luisa, Nanotechnology 26 (2015) 455703
Magnetic phase diagram of superantiferromagnetic TbCu2 nanoparticles C. Echevarria-Bonet, D. P. Rojas, J.I Espeso, J. Rodriguez Fernández, M de la Fuente Rodríguez, L. Fernández Barquín, L. Rodríguez Fenández, P. Gorria, J.A Blanco; Fernandez-Gubieda, Maria Luisa, E. Bauer, F. Damay; J. Phys.: Condensed Matt. 27 (2015) 496002
Interplay between microstructure and magnetism in NiO nanoparticles: breakdown of the antiferromagnetic order, N. Rinaldi–Montes, P. Gorría, D. Martínez–Blanco, J. A. Blanco, L. Fernandez Barquín, J. Rodríguez–Fernández, I. de Pedro, M. L. Fdez–Gubieda and J. Alonso; Nanoscale, 6, (2014) 457-465. Cuartil: 1
Sodium Distribution and Reaction mechanism of a Na3V2O2(PO4)2F electrode during use in a Sodium-ion battery, N. Sharma, P. Serras; V. Palomares, H. Brand, J. Alonso, P. Kubiak, M. L. Fdez-Gubieda, T. Rojo; Chemistry of Materials 26 (2014) 3391−3402
Magnetic Properties and Magnetic Entropy Change in Gd/Ti Multilayers, Andrey V. Svalov, Vladimir V. Vas’kovskiy, Konstantin G. Balymov, Javier Alonso, Mª Luisa Fdez-Gubieda, and Galina V. Kurlyandskaya; IEEE TRANSACTIONS ON MAGNETICS, VOL. 50, No. 11, (2014) 2302204.
Magnetite biomineralization in Magnetospirillum gryphiswaldense: time-resolved magnetic and structural studies, M. Luisa Fdez-Gubieda, Alicia Muela, Javier Alonso, Ana García-Prieto, Luca Olivi, Rodrigo Fernández-Pacheco, and José Manuel Barandiarán, ACS Nano, vol.7, n.4, 3297-3305 (2013)
Magnetostatic interactions in various magnetosome clusters, N. A. Usov, M. L. Fdez-Gubieda, and J. M. Barandiarán, Journal Applied Physics, 113, 023907 (2013)
Size-induced superantiferromagnetism with reentrant spin glass behavior in metallic nanoparticles of TbCu2, C. Echevarria-Bonet, D. P. Rojas, J. I. Espeso, J. Rodríguez Fernández, L. Rodríguez Fernández, P. Gorria, J. A. Blanco, M. L. Fdez-Gubieda, E. Bauer, G. André, and L. Fernández Barquín; Physical Review B Rapid Communication, 87 180407(R) (2013)
Magnetic disorder in diluted FexM100−x granular thin films (M=Au, Ag, Cu; x < 10 at.%), D. Alba Venero, L. Fernández Barquín, J. Alonso, M. L. Fdez-Gubieda, L. Rodríguez Fernández, R. Boada and J. Chaboy; J. Phys.: Condens. Matter 25 (2013) 276001.
The electrochemical Na extraction/insertion of Na3V2O2x(PO4)2F3-2x, Paula Serras, Verónica Palomares, Javier Alonso, Neeraj Sharma, Juan Miguel López del Amo, Pierre Kubiak, María Luisa Fdez-Gubieda and Teófilo Rojo; Chemistry of Materials 25 (2013), 4917-4925.