The combination of cell's microenvironment control and real time monitoring of cell signaling events could provide key biological information. Through precise multipatterning of gold nanoparticles (GNPs) around cells, sensing and actuating elements can be introduced in the cells' microenviroment, providing a powerful substrate for cell studies. In the present work we implemented a combination of techniques to engineer complex substrates for cell studies. Alternating GNPs and bioactive areas were created with micrometer separation, by means of a combination of drying GNPs in microfluidic channels and protein micro-contract printing. In contrast to conventional microfluidics that need syringe pumps to flow liquid in the microchannels, degas driven flow was used to fill dead end channels, rendering the fabrication process straightforward and accessible. We call this new combined technique Printing and Vacuum lithography (PnV lithography). By using different gold nanoparticles with various organic coating ligands, we were able to obtain different macroscale patterns such as wires, supercrystals, and uniformly spread nanoparticle layers that can find different applications depending on the need of the user. We tested the application of the system to pattern a range of mammalian cell lines and obtain readouts on cell viability, cell morphology and presence of cell adhesive proteins.