This paper describes the design, fabrication and performance of a reusable ionogel-based photo-actuator, in-situ photopolymerised into a lab-on-a-disc microfluidic device, for flow control. The ionogel provides an effective barrier to liquids during storage of reagents and spinning of the disc. A simple LED (white light) triggers actuation of the ionogel for selective and precise channel opening at a desired location and time. The mechanism of actuation is reversible, and regeneration of the actuator is possible with an acid chloride solution. In order to achieve regeneration, the Lab-on-a-Disc device was designed with a microchannel connected perpendicularly to the bottom of the ionogel actuator (regeneration channel). This configuration allows the acid solution to reach the actuator, independently from the main channel, which initiates ionogel swelling and main channel closure, and thereby enables reusability of the whole device.