Label-Free Optical Detection of Molecular and Cellular Binding on Reflectionless Glass
- Authors:
- S. Biffi, G. Tagliabue, M. Travagnin, F. Damin, M. Chiari, S. Deegan, M. Tijero, J. Saez, F. Benito-Lopez, M. Salina, M. Buscaglia
- Year:
- 2016
- Presentation in congress:
- 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Micro Total Analysis Systems μ-TAS-2016
- Publishing city and/or Editorial:
- Dublin, Ireland
- Volume:
- 9-13 October
- Initial page - Ending page:
- 39 - 40
- Description:
-
Rapid detection and quantification of molecular and microbiological contaminants in water samples represent common needs of different application fields. In this work we report a novel method enabling the multiplex optical detection of both molecular and micron-sized particle binding on a functionalised glass slide embedded in a microfluidic cell, without using fluorochrome markers nor colorimetric reaction. We characterize the self-assembly of the array of probe proteins via DNA-directed immobilization and demonstrate the detection of micro-particles on the same surface. The proposed method provides an important tool to develop novel arrays for rapid screening of bacteria.