Plant roots exudate certain compounds that have a vital role in the plant-soil interactions, such as sugars, amino acids and mucilage, among others. Root exudation is dependent on several factors, like the plant species, root morphology, the conditions of the soil and the region of the root. However, due to the underground nature of the roots, there is not much information about these plant-soil interactions and the processes involved in root exudation. The development of novel techniques in the field of microfluidics can be useful to gain knowledge about plant exudates and their interactions with soil microorganisms. This study focuses on the growth of tomato and wheat seedlings inside paper-based microfluidics devices. Hydrophobic barriers were created in the paper by using a wax printer. Different wax patterns and paper channel parameters were tested in this work. The different designs were drawn using CorelDraw. For the development of the microfluidic devices, several material combinations were used: PDMS + glass slide, PMMA + PSA and COP + PSA. A colorimetric sensor was inserted in the devices as well, in order to detect glucose exuded by the root.