Cell culture is the principle tool for growing cells out of the organism under controlled conditions in order to study cell fate and behavior. For that, it is necessary to imitate the physiological conditions that occur in a real organism to achieve the most realistic information. Nowadays numerous advancements have been made in order to achieve technologies that can imitate in a real way those physiological conditions. In this article we describe a microfluidic device where thanks to the microcontact printing produces cell patterns that permit controlling from dozens to hundreds of individual cell cultures with total control over the cell interactions with the environment and between the cells. This microfluidic device permits producing controlled physiological conditions for a proper and real cell culture and it also is able to stimulate cells using FGF or monitor their VEGF secretion. This device can be the future cell culture model for the understanding of the cell biology as an alternative for animal testing and offers a high monitoring of cell fate thanks to its ability to stimulate cells and detect their secretion.