Cytotoxicity studies are important for testing the toxicity and efficacy of new compounds in drug development and biomedical research. Microtechnologies such as Organ-on-a-Chip in 3D cultures or the SCADA (Single-Cell Adhesion Dot Array) method in 2D cultures, enable biological studies and cytotoxicity tests in a more reliable, cheaper and faster way than current techniques, as well as they reduce the number of experimental animals and other costs associated to those. The SCADA principle is of interest for our group. It uses microcontact printing to create protein patterns that are used to measure cell adhesion and detachment with single cell resolution. The present paper is divided in two main sections. The first one is a short review of the role of microtechnologies in drug development and how they can help control cell adhesion and cell-cell interaction. The second section relates an experimental work initially designed to further explore the optimization of the SCADA substrates, but rendered shorter than expected due to preventive measures against COVID-19. The influence of several parameters which impact in the quality of SCADA substrates were evaluated. Specifically, air plasma treatment times, substrate material and environmental humidity. The results show that 40 seconds of air plasma render the best results for both, glass and plastic substrates at normal room humidity conditions, but the use of over-dried stamps and glass greatly improves the amount of printed protein on the surface.