Subject
Advanced nanomedicine in metabolic and inflammatory diseases: development and applications
General details of the subject
- Mode
- Face-to-face degree course
- Language
- English
Description and contextualization of the subject
Nanomedicine is the application of nanotechnology to achieve innovation in healthcare. It uses the properties developed by a material at its nanometric scale, which often differ in terms of physics, chemistry or biology from the same material at a bigger scale. Moreover, the nanometric size is also the scale of many biological mechanisms in the human body allowing nanoparticles and nanomaterials to potentially cross natural barriers to access new sites of delivery and to interact with DNA or small proteins at different levels, in blood or within organs, tissues or cells. Thus, nanomedicine has the potential to enable early detection and prevention and to drastically improve diagnosis, treatment and follow-up of many diseases, including metabolic and inflammatory diseases, among others.¿Advanced nanomedicine in metabolic and inflammatory diseases: development and applications¿ covers leading and emerging technologies for nanoparticle preparation, characterization and functionalization. The subject described numerous techniques and procedures for the preparation of nanoparticles of different nature, including organic (lipidic, polymeric, etc) and inorganic (magnetite, silica, gold, etc) ones. The students will be introduced to the methods to functionalize nanoparticles for biomedical applications, including functionalization for specific cell/tissue targeting and drug or RNA delivery. In addition, the most recent biomedical applications of nanoparticles will be described, paying special attention to their use in the diagnosis and treatment of metabolic ad inflammatory diseases.
Teaching staff
Name | Institution | Category | Doctor | Teaching profile | Area | |
---|---|---|---|---|---|---|
BELLOSO URIBE, KEPA | University of the Basque Country | Profesorado Adjunto (Ayudante Doctor/A) | Doctor | Bilingual | Biochemistry and Molecular Biology | kepa.belloso@ehu.eus |
BENITO VICENTE, ASIER | University of the Basque Country | Profesorado Adjunto (Ayudante Doctor/A) | Doctor | Bilingual | Biochemistry and Molecular Biology | asier.benito@ehu.eus |
CASTELLANOS RUBIO, AINARA | University of the Basque Country | Visitante Ikerbaske | Doctor | Not bilingual | Genetics | ainara.castellanos@ehu.eus |
CASTELLANOS RUBIO, IDOIA | University of the Basque Country | Profesorado Adjunto (Ayudante Doctor/A) | Doctor | Bilingual | Inorganic Chemistry | idoia.castellanos@ehu.eus |
GALICIA GARCIA, UNAI | University of the Basque Country | Profesorado Adjunto (Ayudante Doctor/A) | Doctor | Bilingual | Biochemistry and Molecular Biology | unai.galicia@ehu.eus |
JEBARI BENSLAIMAN, SHIFA | University of the Basque Country | Profesorado Ayudante Doctor | Doctor | Bilingual | Biochemistry and Molecular Biology | shifa.jebari@ehu.eus |
LARREA SEBAL, ASIER | University of the Basque Country | Doctor | Bilingual | Teaching of Experimental Sciences | asier.larreas@ehu.eus | |
MARTIN PLAGARO, CESAR AUGUSTO | University of the Basque Country | Profesorado Pleno | Doctor | Bilingual | Biochemistry and Molecular Biology | cesar.martin@ehu.eus |
RUJAS DIEZ, EDURNE | University of the Basque Country | Visitante Ikerbaske | Doctor | Not bilingual | Pharmacy and Pharmaceutical Technology | edurne.rujas@ehu.eus |
SANTIN GOMEZ, IZORTZE | University of the Basque Country | Profesorado Agregado | Doctor | Bilingual | Biochemistry and Molecular Biology | izortze.santin@ehu.eus |
VALCARCEL JIMENEZ, LOREA | University of the Basque Country | Doctor | Not bilingual | ** n o c o n s t a e l a r e a * ó " á r e a p r o v i s i o n a l" | lorea.valcarcel@ehu.eus | |
BLAZQUEZ GARCIA, LOREA | Asociación Instituto Biodonostia | Otros | Doctor | lorea.blazquezgarcia@bio-gipuzkoa.eus |
Study types
Type | Face-to-face hours | Non face-to-face hours | Total hours |
---|---|---|---|
Lecture-based | 20 | 30 | 50 |
Applied laboratory-based groups | 20 | 30 | 50 |
Applied computer-based groups | 10 | 15 | 25 |
Training activities
Name | Hours | Percentage of classroom teaching |
---|---|---|
Experimental practices and report preparation | 50.0 | 40 % |
Lectures | 50.0 | 40 % |
Work with computer equipment and preparation of reports | 25.0 | 40 % |
Assessment systems
Name | Minimum weighting | Maximum weighting |
---|---|---|
Attendance and participation | 50.0 % | 50.0 % |
Evaluation of works/reports | 50.0 % | 50.0 % |
Temary
1.- Introduction to nanomedicine2.- Production and characterization of HDL nanoparticles
3.- Functionalization of HDL nanoparticles for atherosclerosis treatment
4.- Production and characterization of magnetite nanovehicles.
5.- Use of nanoparticles for the treatment of intestinal inflammatory diseases.
6.- Use of nanoparticles for the prevention of diabetes.
7.- Personalized RNA-NPs for the design of therapeutic strategies.
8.- Antibody engineering for therapeutic purposes and the development of lipid nanoparticle platforms for their in vivo delivery.
Laboratory practical lessons
During the laboratory practical lessons, the students will produce and characterize HDL nanoparticles (NPs). In addition, they will functionalize the surface of the nanoparticles with small interfering siRNAs targeting a transcription factor implicated in the activation of inflammation in pancreatic beta cells. To probe the effectiveness of the siRNA-loaded nanoparticles, the student will introduce the siRNA-NPs in the rat INS-1E cell line and analyze the expression of some inflammatory genes by qPCR.
The laboratory practical lessons will allow the students to apply the concepts learned during the theorical lessons.
Bibliography
Compulsory materials
e-Gela platform from the UPV/EHU https://egela.ehu.eus/login/index.phpBasic bibliography
1. Bionanotechnology. Concepts and Applications. Ljiljana Fruk & Antonina Kerbs.Cambridge University Press, 2021.2. Nanotechnology:An Introduction 2ª ed.Jeremy J. Ramsden.Elsevier,2016.
3. Nanotechnology. Understanding small systems.3ª ed.B. Rogers, J. Adams y S. Pennathur. CRC Press, 2015.
4. Understanding Nanomedicine: An Introductory textbook. R. Burgess. Pan Standford Publishing, 2012.
5. Structural DNA Nanotechnology. Nadrian C. Seeman. Cambridge University Press 2016.
6. Introduction to Nanoscience. GL Hornyak, J. Dutta, HF Tibbals y AK Rao. CRC 2008.
7. Fundamentals in Nanotechnology. GL Hornyak, JJ Moore, HF Tibbals y J. Dutta, CRC, 2009.
8. BioNanotechnology. Elisabeth S. Papazoglou y Aravind Parthasarathy. Morgan y Claypol eds, 2007.
9. NANOTECHNOLOG IN BIOLOGY AND MEDICINE: Methods, Devices, and Applications. Tuan Vo-Dinh (ed) CRC 2007
10. Nanobiotechnology. Concepts, Applications and Perspectives. C.M.Niemeyer y C.A. Mirkin(eds.). Wiley
11. & sons 2004.
12. Nanobiotechnology II: More concepts and applications. Chad A. Mirkin and Christof M. Niemeyer (eds) Wiley 2007
13. Nanobiotechnology of Biomimetic membranes. D.T. Martin.Springer 2007
14. Protein Nanotechnology. T. Vo-Dinh. Humana Press 2005.