This course is taught in the third year of the Chemistry degree. It deals with the fundamentals and analytical applications of the different techniques of instrumental analysis. This subject has no prerequisite for enrollment except for having passed Chemistry II. It is closely related to the subject Experimentation in Analytical Chemistry, where the concepts acquired in the subject Analytical Chemistry are developed. Both subjects are totally contextualized, dealing with the different techniques at the same time, with the aim of allowing the students to consolidate the knowledge acquired in the theoretical subject.

Its adequate development requires basic knowledge of General Chemistry as well as basic concepts of other branches of Chemistry.

It is of vital importance for the development of the profession in any field, especially in those where analytical processes are developed.

MODULE COMPETENCES



M02CM04 To know the analytical process and the steps that compose it. To be able to organize, apply and manage the most appropriate methods in each case.



M02CM08 To be able to choose between different instrumental techniques, simple or combined,

for the characterization of chemical substances.



DESCRIPTION:



The objective in this subject is the development of instrumental analytical methods. Therefore, from the methodological point of view, rudimentary trace analysis is taken as a reference. It starts with a general presentation of instrumental analytical systems and finally goes into detail. The most frequent spectroscopic and electrochemical methods will be described. Subsequently, the chromatographic methods of separation (both gases and liquids) and their hybridization to mass spectrometry will be worked on.

Finally, we will focus on chemometrics, especially in the design of experiments and principal component analysis. The knowledge of this course will be applied in the Experimentation in Analytical Chemistry course.

1.- Analytical instrumentation Description of the parts into which an analytical instrument can be divided. Analytical signal. Signal processing. Instrumental characteristics and classification of instrumental analysis techniques.

2.- Trace analysis General methodology of the analysis. Standardization (univariate calibration), recovery (internal and external standards) and tracers. Quality control and quality assurance.

3.-Spectrometric methods Spectrophotometric methods of atomic and molecular analysis. Instrumental configurations. Applications

4.-Electrochemical methods Electrochemical reactions. Electrochemical cells. Classification of electrochemical methods. Instrumental configuration. Applications.

5.- Chromatographic separation methods Fundamentals of chromatographic separation. Instrumental basis of gas chromatography and liquid chromatography. Chromatographic separation methods

6.- Introduction to chemometrics Design of experiments. Principal component analysis. Regression methods.

The basic concepts will be presented in lectures and practical classes, and problems and practical cases related to the subject will be solved.Although the examples are theoretical, they will be based on real cases. The presentations will be with the help of audiovisual resources, but active student participation will be encouraged by promoting open discussions and brainstorming.



In order to ensure the continuous learning of the students, activities will be organized to be carried out individually and in groups, and will have the direct supervision of the teacher (resolution of exercises of greater difficulty, quizzes...). Students will have the support of the teacher and after the activities they will receive a quick feedback to know what they need to improve and to ensure an autonomous and continuous study.

This subject is not just the simple accumulation of new concepts, but the integration and linking of all the concepts. The student will learn all the resources to be used in the professional activity, by examples of different situations.

It is of utmost importance to acquire the skills to validate and discuss all concepts, and therefore all concepts will have to be internalized and applied, with the help of reflection and feedback from the teacher.

In order to asses the 10% of individual work, seminars and some GA/GO may have an activity that counts for the final grade. In that case, it will be noticed in advance.

• Final Assessment System
• Tools and qualification percentages:
  • Written test to be taken (%): 90
  • Individual works (%): 10

WRITTEN EXAM: 90%

There will be two quarterly mid-term exams in the two established exam periods. In order to be able to take the 2nd part it will be necessary to have passed the 1st part (5/10).

There will be a final exam for those who have not passed the subject in the mid-term exams.

All the contents included in the exams must be passed in a balanced way in order to pass the cited exams.

INDIVIDUAL WORKS: 10%

The remaining 10% of the grade of the course will be completed with individual exercises. It will be necessary to obtain a minimum of 5/10 in this section.

In order to waive the call,it is sufficient not to present

The extraordinary call will consist of a written test that will account for 100% of the grade. All the contents included must be passed in a balanced way to pass the exam (5/10).

In order to waive the call,it is sufficient not to present

Fluency in the use of computers, data sheets (Excel or similar) and word processors (Word or similar).

Basic bibliography

D.A. Skoog, F.J. Holler, T.A. Nieman, Principios de análisis instrumental, McGraw-Hill, Madrid, 2001

J.F. Rubinson, K.A. Rubinson, Análisis instrumental, Prentice Hall, México, 2000

F. Rouessac, A. Rouessac, Chemical analysis: Modern instrumentation methods and techniques, Wiley, Chichester, 2000

L. Hernández, C. González, ¿Introducción al Análisis Instrumental¿, 1ª ed., Ariel Ciencia, Barcelona (2002)

K.H. Esbensen. Multivariate data analysis, 5 ed. Camo AB Oslo, 2001

D.C. Harris, Análisis químico cuantitativo, Grupo editorial iberoamericano, México, 2001

In-depth bibliography

K. Danzer, Analytical Chemistry. Theoretical and metrological fundamentals. Springer, Berlin, 2007
R.G. Brereton. Chemometrics, John Wiley & Sons, Chichester, 2003
R. Kellner, J.-M- Mermet, M. Otto, H.M. Widmer, Analytical Chemistry, Wiley-VCH, Weinheim (1998)
J.K. Taylor, Quality assurance of chemical measurements, Lewis, Michigan, USA, 1987
M. Alley The Craft of Scientific Presentations: Critical Steps to Succeed and Critical Errors to Avoid, Springer Verlag, 2003
Colin F. Poole, The Essence of Chromatography, Elsevier, 2003
R. Cela, R. A. Lorenzo, Mª del Carmen Casais, Técnicas de Separación en Química Analítica, ed. Sintesis, 2002
C.H. Hamann, A. Hamnett, W. Vielstich, Electrochemistry, Wiley-VCH, 1998

Journals

Analytical Chemistry
Trends in Analytical Chemistry
The Analyst
Analytica Chimica Acta
Talanta
Analytical and Bioanalytical Chemistry
LC/GC
Spectroscopy Europe

Web addresses

UPV/EHU library:
http://www.biblioteka.ehu.es/p207-home/eu/
The Analytical Chemistry Springboard:
http://www.anachem.umu.se/jumpstation.htm
International Union of Pure and Applied Chemistry (IUPAC): http://iupac.chemsoc.org
Laboratory of the Government Chemist (LGC) :
http:// www.lgc.co.uk
The Virtual classroom:
http://ull.chemistry.uakron.edu/classroom
The Chemistry Hypermedia Project:
http://www.files.chem.vt.edu/chem-ed/vt-chem-ed.html
Eurachem: http://www.eurachem.org/
Chrom Academy:
http://www.chromacademy.net/