Subject
Cellular-Molecular Biology of Membranes
General details of the subject
- Mode
- Face-to-face degree course
- Language
- English
Description and contextualization of the subject
The course aims to present the current state of knowledge about biological membranes in an accessible and balanced way, paying more attention to the most current aspects and taking advantage of the existence at the UPV/EHU of notable experts in the area, especially in membrane biophysics.All the cells, prokaryotic and eukaryotic, and quite a few viruses, are surrounded by a membrane that delimits them. This membrane always has the same basic structure: a double layer of phospholipids and a series of proteins attached to the lipids. The thickness of the membrane is about 50 nanometres (50 millionths of a millimetre), so these biological objects enter the nanoscience domain. The functions of the membranes are multiple, not only do they serve as a selective barrier for the entry of nutrients and the elimination of toxins, they are also the seat of receptors for hormones and other metabolic control signals, they maintain the mechanisms for controlling cell growth, they prevent infection or invasion by other cells, and, paradoxically, they are also the route that some viruses (influenza, AIDS) take for their entry.
This subject combines three types of lessons with two complementary approaches. There are lessons (or rather working sessions) dedicated to the general review of the field, others more specific in which specific aspects of special relevance are dealt with, and finally others of a methodological nature. Crosswise, the basic biology approach is combined with the biomedical approach.
The course is presented in the context of "Cellular-Molecular Biology". This means that the traditional division between cell biology and molecular biology is transcended to integrate these two disciplines. The methodology of molecular biology allows in many cases to overcome barriers that seemed insurmountable for cell biology, and this is particularly true in the field of biomembranes. It is no less true, on the other hand, that cell biology poses problems and offers integration mechanisms that traditionally escaped the purely molecular level.
Teaching staff
Name | Institution | Category | Doctor | Teaching profile | Area | |
---|---|---|---|---|---|---|
ALONSO IZQUIERDO, ALICIA | University of the Basque Country | Profesorado Catedratico De Universidad | Doctor | Not bilingual | Biochemistry and Molecular Biology | alicia.alonso@ehu.eus |
COLOM DIEGO, ADAI | University of the Basque Country | Investigador Ramón Y Cajal | Doctor | Not bilingual | ** n o c o n s t a e l a r e a * ó " á r e a p r o v i s i o n a l" | adai.colom@ehu.eus |
CONTRERAS GOMEZ, XABIER | University of the Basque Country | Visitante Ikerbaske | Doctor | Not bilingual | Biochemistry and Molecular Biology | xabier.contreras@ehu.eus |
ETXANIZ IRIONDO, ASIER | University of the Basque Country | Profesorado Adjunto (Ayudante Doctor/A) | Doctor | Bilingual | Biochemistry and Molecular Biology | asier.echaniz@ehu.eus |
JIMENEZ ROJO, NOEMI | University of the Basque Country | Doctor | Not bilingual | ** n o c o n s t a e l a r e a * ó " á r e a p r o v i s i o n a l" | noemi.jimenez@ehu.eus | |
LANDETA DIAZ, OLATZ | University of the Basque Country | Profesorado Agregado | Doctor | Bilingual | Biochemistry and Molecular Biology | olatz.landeta@ehu.eus |
MONTES BURGOS, LIDIA RUTH | University of the Basque Country | Profesorado Titular De Universidad | Doctor | Bilingual | Biochemistry and Molecular Biology | lidiaruth.montes@ehu.eus |
NIETO GARAI, JON ANDER | University of the Basque Country | Profesorado Adjunto (Ayudante Doctor/A) | Doctor | Bilingual | Biochemistry and Molecular Biology | jonander.nieto@ehu.eus |
RUIZ MIRAZO, KEPA | University of the Basque Country | Personal Doctor Investigador | Doctor | Not bilingual | Logic and Philosophy of Science | kepa.ruiz-mirazo@ehu.eus |
SHNYROVA ZHADAN, ANNA | University of the Basque Country | Personal Doctor Investigador | Doctor | Not bilingual | Biochemistry and Molecular Biology | anna.shnyrova@ehu.eus |
TERRONES URIO, OIHANA | University of the Basque Country | Profesorado Agregado | Doctor | Bilingual | Biochemistry and Molecular Biology | oihana.terrones@ehu.eus |
Study types
Type | Face-to-face hours | Non face-to-face hours | Total hours |
---|---|---|---|
Lecture-based | 25 | 38 | 63 |
Seminar | 25 | 37 | 62 |
Ordinary call: orientations and renunciation
The evaluation of the course will be based on the following aspects:1. Attendance and participation in class 50% of the final grade. 80% class attendance is required.
2. Scientific report 50% of the final grade.
This method of assessment could be changed if the guidelines of the health authorities so provide. The appropriate modifications would be announced in due course, with the necessary strategies and tools to guarantee the right of the student to be evaluated with equity and fairness.
Extraordinary call: orientations and renunciation
Written proof of the entire course program.This method of assessment could be changed if the guidelines of the health authorities so provide. The appropriate modifications would be announced in due course, with the necessary strategies and tools to guarantee the right of the student to be evaluated with equity and fairness.
Temary
1.INTRODUCTION: RECENT ADVANCES IN MEMBRANE STUDIES.2.THE ORIGIN OF CELL MEMBRANES
3.BIOGENESIS OF CELL MEMBRANES
4.EXOSOMES IN METABOLISM AND PRECISION MEDICINE
5.DEALING WITH MEMBRANE PROTEINS: THEIR PURIFICATION AND RECONSTITUTION.
6.DETERGENTS: WASHING THE DISHES AND BEYOND
7.CRYO-ELECTRON MICROSCOPY: EXAMINING INTACT BIOLOGICAL MEMBRANES.
8.INTERORGANELLE MEMBRANE CONTACT SITES
9.LIPOSOMES AND OTHER MEMBRANE MODELS
10.MEMBRANE-BASED SYSTEMS FOR DRUG AND GENE DELIVERY
11.FLUORESCENCE IMAGING: EXPLORING THE FRONTIERS OF A RAPIDLY DEVELOPING
TECHNIQUE.
12.SUB-CELLULAR ARCHITECTURE STUDIED BY CORRELATIVE LIGHT AND ELECTRON MICROSCOPY.
13.LIPIDOMIC STUDIES OF CELL MEMBRANES.
14.CELL INFECTION BY HIV: STUDYING LIPID ROLES IN HIV LIFE CYCLE
15.STRENGTHENING PROTEIN-MEMBRANE INTERACTIONS: A NOVEL STRATEGY TO
ENHANCE THE POTENCY OF ANTI-HIV ANTIBODIES.
16.MEMBRANE BIOMECHANICS
17.MECHANOADAPTATION AND CAVEOLAE BIOLOGY
18.CELL AUTOPHAGY: MOLECULAR BASIS FOR THE AUTOPHAGOSOME GENERATION
19.THE MOLECULAR MECHANISMS OF APOPTOSIS.
20. MEMBRANE FISSION AND CURVATURE-SENSING PROTEINS
21.ENDOSOMAL TRAFFIC AT ATOMIC RESOLUTION
22.OPTOGENETIC CONTROL OF MEMBRANE FLUIDITY AND SECRETION
23.MEMBRANE STUDIES APPLIED TO CANCER THERAPEUTICS
Bibliography
Compulsory materials
For each session included in the programme, teachers will provide the student with additional teaching materials (i.e. PowerPoint presentations and other specialised references such as reviews or research articles) using the Egela digital platform.Basic bibliography
The Biophysics of Cell Membranes. Biological Consequences. Editors: Epand, Richard M., Ruysschaert, Jean-Marie (Eds.) Springer, New York. (2017) ISBN 978-981-10-6244-5The Structure of Biological Membranes (3rd ed.) Philip Y. Yeagle. CRC Press, Boca Raton FL, (2011). ISBN: 9781439809570
Cell Membranes . Lukas Buehler. Garland Science, New York. (2015) ISBN: 978-0815341963.
Membrane Structural Biology with Biochemical and Biophysical Foundations (2nd ed.). Mary Luckey. Garland Science, New York. (2022). ISBN:978-0393884852 .
Molecular Biology of the Cell (7th ed.) Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter (eds.) . Garland Science, New York. (2014). ISBN: 9780815344322
Membrane Protein Crystallization and Purification (2nd ed.). Carola Hunte, Gebhard von Jagow and Hermann Schagger (eds). Academic Press, Amsterdam. (2003). ISBN: 0-12-361776-6